6-磷酸葡萄糖脱氢酶（大肠杆菌）150单位 爱尔兰 型号:JKY/E-PGDHEC 库号：M230023 外观：悬浮液
在水中的溶解性：易溶。
pH值：6.0
气味：无
状态：液体
保质期：在冰箱5年或以上
成分：
名称 比例
6磷酸葡萄糖脱氢酶 1％
硫酸铵 45％
叠氮钠 0.02％
水 54％
EC 1.1.1.44. Recombinant from E. coli. In 3.2 M ammonium sulphate. Specific activity: 21.4 U/mg (25oC, pH 7.6). Contaminants: NADH oxidase = 0.00015 %, NADPH oxidase < 0.001 %. Stable at 4oC for > 2 years.
PROPERTIES
1. ELECTROPHORETIC PURITY
- Single band on SDS-gel electrophoresis (MW ~ 52,546)
- Single major band on isoelectric focusing (pI ~ 5.3)
2. SPECIFIC ACTIVITY AND LEVEL OF OTHER ACTIVITIES
21.4 U/mg protein at pH 7.6 and 25°C.
One Unit of 6-phosphogluconate dehydrogenase (6-PGDH) is defined as the amount
of enzyme required to produce one µmole of NADPH from NADP+
under the
following assay conditions:
TEA buffer, pH 7.6 86 mM
ATP 7.5 mM
MgCl
2 8.6 mM
D-Gluconic acid 3.2 mM
NADP+ 0.9 mM
Gluconate kinase 6.0 U/mL
3. OTHER ACTIVITIES (as a percentage of 6-PGDH activity)
Enzyme measured Substrate Activity, %
6-PGDH D-gluconate 6-phosphate 100
NADH oxidase NADH 0.00015
NADPH oxidase NADPH 0.000000
4. PHYSICOCHEMICAL PROPERTIES:
Recommended conditions of use are at pH 7.6 and up to 25°C.
5. STORAGE AND USE CONDITIONS/RECOMMENDATIONS:
The enzyme is supplied as an ammonium sulphate suspension and should be stored at
4°C. Swirl to mix the enzyme suspension immediay prior to use.中 |
